PCR Supplies
INTACT GENOMICS INC igScript™ Probe-Based One Step RT-qPCR Kit 500 Reactions
IgScript™ probe-based RT-qPCR kit combines i) IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix for high efficiency, wide dynamic range, sensitivity, and specificity. The cDNA kit includes 5x IgScript™ master mix (Reverse Transcriptase, RNase inhibitor, dNTPs, buffer, MgCl₂, stabilizers), plus oligo-dT [d(T)23VN] and random hexamer primers for complete coverage of mRNA and non-polyadenylated RNAs. IgScript™ Reverse Transcriptase is a recombinant MMLV enzyme with reduced RNase H activity and higher thermostability, enabling full-length cDNA synthesis at 42–50 ºC. The qPCR 2x master mix (excluding primers/template) contains proprietary buffer, hot start Taq polymerase, low ROX dye, MgCl₂, dNTPs, stabilizers, and enhancers for robust amplification.Applications: Gene expression validation, multiplexing, mutation detection, pathogen/viral detection, GMO characterization, profiling.
INTACT GENOMICS INC igScript™ One Step RT-PCR Kit 100 Reactions
igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.Applications: Gene expression data validation. Multiplexing Mutation detection Pathogen and viral detection Genetically modified organisms (GMO) characterization and Genetic profiling Benefits: Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55°C.
INTACT GENOMICS INC Taq DNA Polymerase 1000 Units
Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Applications Routing PCR cloning Primer extension Colony PCR Elongation efficiency 1.0-1.2 kb/min. Formulated for amplifying long target DNA. Efficient for amplifying high GC content DNA with Intact Genomics magic enhancer
Sino Biological Cynomolgus / Rhesus Transferrin Receptor / TFRC / CD71 Protein (His Tag), Endotoxin-Free 100µg
A DNA sequence encoding the cynomolgus/rhesus TFRC (XP_005545315.1 & NP_001244232.1) (Cys89-Phe760) was expressed with a polyhistidine tag at the N-terminus. Cynomolgus and Rhesus TFRC sequences are identical.
LIFE TECHNOLOGIES TAQMAN QSYPROBE6 000PMOLES10
44-827-77 TAQMAN QSYPROBE6 000PMOLES10
LIFE TECHNOLOGIES TAQMAN QSYPROBE20 000PMOLES20
44-827-78 TAQMAN QSYPROBE20 000PMOLES20
LIFE TECHNOLOGIES TQMNTAMRAPROBE50 000PMOLESEACH
45-000-3 TQMNTAMRAPROBE50 000PMOLESEACH
New England Biolabs, Inc. Bst 2.0 WarmStart DNA Polymerase – 8000 units
Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45C and therefore no special activation step is needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand-displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
New England Biolabs, Inc. Bst 3.0 DNA Polymerase – 1600 units
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5' to 3' DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5' to 3' and 3' to 5' exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 250 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
Cytek Biosciences Inc CYBRF 1STEP RTQPCR LOROX 300R
CYBRFast 1-Step RT-qPCR Lo-ROX Kit Apps: RT-qPCR Size: 300 rxns